Dosage suppressors of the dominant G1 cyclin mutant CLN3-2: identification of a yeast gene encoding a putative RNA/ssDNA binding protein.

Three G1 cyclins, CLN1, CLN2, and CLN3, have been identified in the budding yeast Saccharomyces cerevisiae. G1 cyclins are essential, albeit functionally redundant, rate-limiting activators of cell cycle initiation. We have isolated dosage-dependent suppressor genes (designated HMD genes) of the mating defect caused by CLN3-2, a dominant mutation in CLN3, ...
HMD2 and HMD3 are identical to STE4 and STE5, respectively, HMD1 is an essential gene that encodes a protein containing a putative RNA binding domain. Overproduction of HMD1 results in a relatively specific reduction in the level of the CLN3 or CLN3-2 transcript. This reduction occurs subsequent to transcription initiation of CLN3 since overexpression of HMD1 did not affect expression of a heterologous transcript from the CLN3 promoter but did result in a reduction of CLN3 transcript expressed from a heterologous promoter. HMD1 has at least one essential role independent of its effect on CLN3 since HMD1 remains essential for viability in the absence of a functional CLN3 gene.
Mesh Terms:
Amino Acid Sequence, Cell Division, Cyclins, DNA, Single-Stranded, DNA-Binding Proteins, Fungal Proteins, G1 Phase, Gene Expression Regulation, Fungal, Genes, Dominant, Genes, Fungal, Genes, Suppressor, Genotype, Molecular Sequence Data, Nuclear Proteins, RNA, Fungal, RNA-Binding Proteins, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Analysis, Transcription, Genetic
Mol. Gen. Genet.
Date: Oct. 25, 1995
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