The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results (Pre-Publication)

Harvard Medical School Department of Cell Biology
As part of an ongoing collaboration between the labs of Steve Gygi and Wade Harper in the Department of Cell Biology at Harvard Medical School, we are profiling systematically the interactions among human proteins using affinity purification mass spectrometry. HA-tagged baits taken from the human ORFeome (version 8.1; Marc Vidal) have been expressed individually in HEK 293T cells, followed by affinity purification mass spectrometry to identify interacting partners that are specific to each bait. Interacting proteins have been identified using the CompPASS algorithm (Sowa et all Cell 2009) with modifications for high-throughput data processing. While this study is ongoing, we first reported an initial version of the BioPlex network including 23,744 interactions from the first 2594 AP-MS experiments (Huttlin et al. Cell 2015 - PMID 26186194). We have since published an updated network, termed BioPlex 2.0 (Huttlin et al. Nature 2017 - PMID 28514442), that includes 5891 AP-MS experiments and produces a network of 56,553 interactions spanning 10,961 proteins. BioPlex 2.0 includes a complete reanalysis of all data from BioPlex 1.0 as well as 3297 additional AP-MS experiments. Both BioPlex 1.0 and 2.0 have been deposited in BioGRID (published dataset identifiers and links). To date, and additional 17,338 interactions from 1612 AP-MS experiments have been identified, beyond those that have been published. These interactions are included in this dataset, which will be updated periodically as additional data become available.
Status: Pre-Publication Dataset
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