Sro7 and Sro77, the yeast homologues of the Drosophila lethal giant larvae (lgl), regulate cell proliferation via Rho1-Tor1 pathway.

The Saccharomyces cerevisiae Sro7 and Sro77 are homologues to the Drosophila tumor suppressor lethal giant larvae (lgl), which regulates cell polarity in Drosophila epithelial cells. We show that double mutation of SRO7/SRO77 is defective in colony growth. The colony of SRO7/SRO77 double deletion is much smaller than the wild type ...
and appears to be round with a smooth surface, compared to the wild type. Analysis using transmission electron microscopy revealed multiple defects of the colony cells, including multi-budding, multi-nuclei, cell lysis and dead cells, suggesting that the double deletion causes defects in cell polarity and cell wall integrity (CWI). Over-expression of RHO1, one of the central regulators of cell polarity and CWI, fully recovered the sro7Δ/sro77Δ phenotype. We further demonstrate that sro7Δ/sro77Δ causes a decrease of the GTP-bound, active Rho1, which in turn, causes an up-regulation of TOR1. Deletion of TOR1 in sro7Δ/sro77Δ (sro7Δ/sro77Δ/tor1Δ) recovered the cell growth and colony morphology, similar to wild type. Our results suggest that the tumor suppressor homologue SRO7/SRO77 regulates cell proliferation and yeast colony development via the Rho1-Tor1 pathway.
Microbiology (Reading, Engl.)
Date: Jul. 24, 2014
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