Differential phosphorylation of c-Abl in cell cycle determined by cdc2 kinase and phosphatase activity.
The product of the c-abl proto-oncogene (c-Abl) is phosphorylated on three sites during interphase and seven additional sites during mitosis. Two interphase and all mitotic c-Abl sites are phosphorylated by cdc2 kinase isolated from either interphase or mitotic cells, with the mitotic cdc2 having an 11-fold higher activity. Inhibition of ... phosphatases with okadaic acid in interphase cells leads to the phosphorylation of c-Abl mitotic sites, indicating that those sites are preferentially dephosphorylated during interphase. The differential phosphorylation of c-Abl in the cell cycle is therefore determined by an equilibrium between cdc2 kinase and protein phosphatase activities. Treatment of interphase cells with okadaic acid leads to a rounded morphology similar to that observed during mitosis.
Mesh Terms:
Amino Acid Sequence, Animals, CDC2 Protein Kinase, Cell Cycle, Cells, Cultured, Ethers, Cyclic, Interphase, Mice, Mice, Inbred Strains, Molecular Sequence Data, Molecular Weight, Okadaic Acid, Peptide Fragments, Peptide Mapping, Phosphoproteins, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-abl
Amino Acid Sequence, Animals, CDC2 Protein Kinase, Cell Cycle, Cells, Cultured, Ethers, Cyclic, Interphase, Mice, Mice, Inbred Strains, Molecular Sequence Data, Molecular Weight, Okadaic Acid, Peptide Fragments, Peptide Mapping, Phosphoproteins, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-abl
Science
Date: Apr. 13, 1990
PubMed ID: 2183353
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