Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae.

Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream ...
protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.
Mesh Terms:
1-Phosphatidylinositol 4-Kinase, Alkaloids, Amino Acid Sequence, Base Sequence, Cell Cycle, DNA, Fungal, Drug Resistance, Microbial, Genes, Fungal, Genes, Suppressor, Molecular Sequence Data, Mutation, Phenotype, Phosphotransferases (Alcohol Group Acceptor), Protein Kinase C, Protein Kinases, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Staurosporine, Temperature
Mol. Gen. Genet.
Date: Mar. 01, 1994
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