Acidic di-leucine motif essential for AP-3-dependent sorting and restriction of the functional specificity of the Vam3p vacuolar t-SNARE.
The transport of newly synthesized proteins through the vacuolar protein sorting pathway in the budding yeast Saccharomyces cerevisiae requires two distinct target SNAP receptor (t-SNARE) proteins, Pep12p and Vam3p. Pep12p is localized to the pre-vacuolar endosome and its activity is required for transport of proteins from the Golgi to the ... vacuole through a well defined route, the carboxypeptidase Y (CPY) pathway. Vam3p is localized to the vacuole where it mediates delivery of cargoes from both the CPY and the recently described alkaline phosphatase (ALP) pathways. Surprisingly, despite their organelle-specific functions in sorting of vacuolar proteins, overexpression of VAM3 can suppress the protein sorting defects of pep12Delta cells. Based on this observation, we developed a genetic screen to identify domains in Vam3p (e.g., localization and/or specific protein-protein interaction domains) that allow it to efficiently substitute for Pep12p. Using this screen, we identified mutations in a 7-amino acid sequence in Vam3p that lead to missorting of Vam3p from the ALP pathway into the CPY pathway where it can substitute for Pep12p at the pre-vacuolar endosome. This region contains an acidic di-leucine sequence that is closely related to sorting signals required for AP-3 adaptor-dependent transport in both yeast and mammalian systems. Furthermore, disruption of AP-3 function also results in the ability of wild-type Vam3p to compensate for pep12 mutants, suggesting that AP-3 mediates the sorting of Vam3p via the di-leucine signal. Together, these data provide the first identification of an adaptor protein-specific sorting signal in a t-SNARE protein, and suggest that AP-3-dependent sorting of Vam3p acts to restrict its interaction with compartment-specific accessory proteins, thereby regulating its function. Regulated transport of cargoes such as Vam3p through the AP-3-dependent pathway may play an important role in maintaining the unique composition, function, and morphology of the vacuole.
Mesh Terms:
Adaptor Proteins, Vesicular Transport, Alkaline Phosphatase, Biological Transport, Carboxypeptidases, Cathepsin A, Endosomes, Fungal Proteins, Leucine, Membrane Proteins, Monomeric Clathrin Assembly Proteins, Mutation, Nerve Tissue Proteins, Phosphoproteins, Qa-SNARE Proteins, SNARE Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Vesicle-Associated Membrane Protein 3, Vesicular Transport Proteins
Adaptor Proteins, Vesicular Transport, Alkaline Phosphatase, Biological Transport, Carboxypeptidases, Cathepsin A, Endosomes, Fungal Proteins, Leucine, Membrane Proteins, Monomeric Clathrin Assembly Proteins, Mutation, Nerve Tissue Proteins, Phosphoproteins, Qa-SNARE Proteins, SNARE Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Vesicle-Associated Membrane Protein 3, Vesicular Transport Proteins
J. Cell Biol.
Date: Aug. 24, 1998
PubMed ID: 9722605
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