A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae.

The PKC1-MPK1 pathway in yeast functions in the maintenance of cell wall integrity and in the stress response. We have identified a family of genes that are putative regulators of this pathway. WSC1, WSC2, and WSC3 encode predicted integral membrane proteins with a conserved cysteine motif and a WSC1-green fluorescence ...
protein fusion protein localizes to the plasma membrane. Deletion of WSC results in phenotypes similar to mutants in the PKC1-MPK1 pathway and an increase in the activity of MPK1 upon a mild heat treatment is impaired in a wscDelta mutant. Genetic analysis places the function of WSC upstream of PKC1, suggesting that they play a role in its activation. We also find a genetic interaction between WSC and the RAS-cAMP pathway. The RAS-cAMP pathway is required for cell cycle progression and for the heat shock response. Overexpression of WSC suppresses the heat shock sensitivity of a strain in which RAS is hyperactivated and the heat shock sensitivity of a wscDelta strain is rescued by deletion of RAS2. The functional characteristics and cellular localization of WSC suggest that they may mediate intracellular responses to environmental stress in yeast.
Mesh Terms:
Amino Acid Sequence, Cell Wall, Cyclic AMP, Fungal Proteins, Gene Deletion, Genes, Fungal, Genes, ras, Hot Temperature, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Mitogen-Activated Protein Kinases, Molecular Sequence Data, Multigene Family, Mutation, Phenotype, Protein Kinase C, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid
Proc. Natl. Acad. Sci. U.S.A.
Date: Dec. 09, 1997
Download Curated Data For This Publication
16776
Switch View:
  • Interactions 10