A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control.
Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts- mutants display a G2/M ... cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30Delta mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable Rsc3/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC.
Mesh Terms:
Base Sequence, Cell Cycle, Cell Wall, Chromatin, DNA-Binding Proteins, Dimerization, G2 Phase, Gene Expression Regulation, Fungal, Genes, Lethal, Genes, cdc, Leucine Zippers, Mitosis, Mitotic Spindle Apparatus, Molecular Sequence Data, Mutagenesis, Site-Directed, Nuclear Proteins, Protein Structure, Secondary, Protein Structure, Tertiary, Ribosomal Proteins, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factors, Yeasts, Zinc
Base Sequence, Cell Cycle, Cell Wall, Chromatin, DNA-Binding Proteins, Dimerization, G2 Phase, Gene Expression Regulation, Fungal, Genes, Lethal, Genes, cdc, Leucine Zippers, Mitosis, Mitotic Spindle Apparatus, Molecular Sequence Data, Mutagenesis, Site-Directed, Nuclear Proteins, Protein Structure, Secondary, Protein Structure, Tertiary, Ribosomal Proteins, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factors, Yeasts, Zinc
Mol. Cell
Date: Apr. 01, 2001
PubMed ID: 11336698
View in: Pubmed Google Scholar
Download Curated Data For This Publication
16853
Switch View:
- Interactions 12