Interaction of human leukotriene C4 synthase and microsomal glutathione transferase in vivo.

Microsomal glutathione transferase (mGT) specifically binds leukotriene C4 synthase in the presence of Mg2+ ion (Soederstroem et al., Protein Expression and Purification (1995) 6, 352-356). To investigate if this interaction occurs in vivo we screened a human lung cDNA library with a bait vector encoding human mGT in the yeast ...
two-hybrid system. One of the five positive clones obtained encoded leukotriene C4 synthase. This clone was expressed in two heterologous systems. The recombinant protein cross-reacted with a guinea pig antibody raised against a Keyhole limpet hemocyanin coupled synthetic peptide corresponding to amino acids 141-150 of human leukotriene C4 synthase.
Mesh Terms:
Amino Acid Sequence, Base Sequence, DNA, Complementary, Glutathione Transferase, Humans, Lung, Microsomes, Molecular Sequence Data
Biochem. Biophys. Res. Commun.
Date: Dec. 13, 1996
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