Next-generation sequencing to generate interactome datasets.

Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to ...
generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.
Mesh Terms:
Databases, Protein, Humans, Open Reading Frames, Polymerase Chain Reaction, Protein Interaction Mapping, Sequence Analysis, DNA, Two-Hybrid System Techniques
Nat. Methods
Date: Jun. 01, 2011
Download Curated Data For This Publication
Switch View:
  • Interactions 1,166