FIP200 regulates targeting of Atg16L1 to the isolation membrane.
Autophagosome formation is a dynamic process that is strictly controlled by autophagy-related (Atg) proteins. However, how these Atg proteins are recruited to the autophagosome formation site or autophagic membranes remains poorly understood. Here, we found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the ... downstream Atg factors, in an Atg14- and phosphatidylinositol 3-kinase-independent manner. Atg16L1 deletion mutants, which lack the FIP200-interacting domain, are defective in proper membrane targeting. Thus, FIP200 regulates not only early events but also late events of autophagosome formation through direct interaction with Atg16L1.
Mesh Terms:
Animals, Autophagy, Carrier Proteins, Cell Membrane, Gene Deletion, HEK293 Cells, Humans, Intracellular Signaling Peptides and Proteins, Mice, Phosphatidylinositol 3-Kinase, Protein Binding, Protein Interaction Domains and Motifs, Protein Transport, Vesicular Transport Proteins
Animals, Autophagy, Carrier Proteins, Cell Membrane, Gene Deletion, HEK293 Cells, Humans, Intracellular Signaling Peptides and Proteins, Mice, Phosphatidylinositol 3-Kinase, Protein Binding, Protein Interaction Domains and Motifs, Protein Transport, Vesicular Transport Proteins
EMBO Rep.
Date: Mar. 01, 2013
PubMed ID: 23392225
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