Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton.

The yeast tropomyosin gene, designated TPM1, is present in a single copy per haploid genome and encodes a protein with a predicted molecular weight of 23.5 kd. The protein sequence is homologous to higher cell tropomyosins, including the characteristic hydrophobic-hydrophilic pseudoheptapeptide repeats. Indirect immunofluorescence microscopy reveals that tropomyosin is localized ...
with actin cables in wild-type cells. Disruption of TPM1 is not lethal, but results in a reduced growth rate and disappearance of actin cables. Strains carrying the conditional actin mutation act1-2 also lack actin cables; overexpression of tropomyosin in these strains partially restores actin cables. These results strongly suggest that tropomyosin interacts with F actin in vivo and may play an important role in assembling or stabilizing actin cables in yeast.
Mesh Terms:
Actins, Base Sequence, Cytoskeleton, Gene Expression Regulation, Genes, Genes, Lethal, Molecular Sequence Data, Saccharomyces cerevisiae, Tropomyosin
Cell
Date: Apr. 21, 1989
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