Isolation of UBP3, encoding a de-ubiquitinating enzyme, as a multicopy suppressor of a heat-shock mutant strain of S. cerevisiae.

Yeast strains lacking functional copies of the two genes SSA1 and SSA2, which encode cytosolic molecular chaperones, are temperature-sensitive. In this report, we describe the isolation of a high-copy suppressor of this temperature sensitivity, UBP3, which encodes a de-ubiquitinating enzyme. We show that ubp3 mutant yeast strains have a mild ...
slow-growth phenotype and accumulate ubiquitin-protein conjugates. We propose a model in which Ubp3p acts in vivo to reverse the ubiquitination of substrate proteins, allowing temporarily misfolded proteins an opportunity to fold correctly.
Mesh Terms:
Adenosine Triphosphatases, Cell Division, Endopeptidases, Fungal Proteins, Gene Dosage, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Suppressor, HSP70 Heat-Shock Proteins, Heat-Shock Proteins, Mutagenesis, Insertional, Mutation, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Transcription, Genetic, Ubiquitins
Curr. Genet.
Date: Jun. 01, 1998
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