Isolation of UBP3, encoding a de-ubiquitinating enzyme, as a multicopy suppressor of a heat-shock mutant strain of S. cerevisiae.
Yeast strains lacking functional copies of the two genes SSA1 and SSA2, which encode cytosolic molecular chaperones, are temperature-sensitive. In this report, we describe the isolation of a high-copy suppressor of this temperature sensitivity, UBP3, which encodes a de-ubiquitinating enzyme. We show that ubp3 mutant yeast strains have a mild ... slow-growth phenotype and accumulate ubiquitin-protein conjugates. We propose a model in which Ubp3p acts in vivo to reverse the ubiquitination of substrate proteins, allowing temporarily misfolded proteins an opportunity to fold correctly.
Mesh Terms:
Adenosine Triphosphatases, Cell Division, Endopeptidases, Fungal Proteins, Gene Dosage, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Suppressor, HSP70 Heat-Shock Proteins, Heat-Shock Proteins, Mutagenesis, Insertional, Mutation, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Transcription, Genetic, Ubiquitins
Adenosine Triphosphatases, Cell Division, Endopeptidases, Fungal Proteins, Gene Dosage, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Suppressor, HSP70 Heat-Shock Proteins, Heat-Shock Proteins, Mutagenesis, Insertional, Mutation, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Transcription, Genetic, Ubiquitins
Curr. Genet.
Date: Jun. 01, 1998
PubMed ID: 9644204
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