Genetic interactions link ARF1, YPT31/32 and TRS130.

A genetic screen for synthetic lethal interactions with arf1(-) identified a recessive mutation in TRS130, one of 10 components in the trafficking protein particle (TRAPP) complex (Sacher et al., 2000). As TRS130 is an essential gene, the synthetic lethal allele (trs130-101) is a novel one that requires ARF1 for viability. ...
This allele was found to exhibit no defects in secretory function, i.e. processing of carboxypeptidase Y or invertase. YPT31 and YPT32 were identified in a subsequent screen as high-copy suppressors of arf1(-)trs130-101. Increasing the gene dosage of YPT31/32 also suppressed lethality resulting from deletion of TRS130 or TRS120 but not three other essential TRAPP subunit-encoding genes. Although unable to suppress defects in several alleles of ARF1, increasing the gene dosage of YPT31/32 suppressed the cold sensitivity of gcs1(-), an Arf GTPase-activating protein (GAP). Thus, these genetic interactions provide initial evidence for linkage of Arf and TRAPP signalling and for Ypt31/32 proteins functioning downstream of both components in the TRAPP complex and of Arf signalling via the Gcs1 Arf GAP.
Mesh Terms:
ADP-Ribosylation Factor 1, Alleles, Carrier Proteins, DNA-Binding Proteins, Fungal Proteins, GTPase-Activating Proteins, Gene Dosage, Gene Expression Regulation, Fungal, Genes, Suppressor, Glycoside Hydrolases, Membrane Proteins, Mutation, Saccharomyces cerevisiae Proteins, Signal Transduction, Vesicular Transport Proteins, Yeasts, beta-Fructofuranosidase, rab GTP-Binding Proteins
Yeast
Date: Sep. 15, 2002
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