Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription.

Transcription of the four yeast histone gene pairs (HTA1-HTB1, HTA2-HTB2, HHT1-HHF1, and HHT2-HHF2) is repressed during G1, G2, and M. For all except HTA2-HTB2, this repression requires several trans-acting factors, including the products of the HIR genes, HIR1, HIR2, and HIR3. ASF1 is a highly conserved protein that has been ...
implicated in transcriptional silencing and chromatin assembly. In this analysis, we show that HIR1 interacts with ASF1 in a two-hybrid analysis. Further, asf1 mutants, like hir mutants, are defective in repression of histone gene transcription during the cell cycle and in cells arrested in early S phase in response to hydroxyurea. asf1 and hir1 mutations also show very similar synergistic interactions with mutations in cac2, a subunit of the yeast chromatin assembly factor CAF-I. The results suggest that ASF1 and HIR1 function in the same pathway to create a repressive chromatin structure in the histone genes during the cell cycle.
Mesh Terms:
Blotting, Western, Cell Cycle, Cell Cycle Proteins, Cell Nucleus, Cell Separation, Chromatin, Chromatin Assembly Factor-1, Chromosomal Proteins, Non-Histone, Chromosome Deletion, DNA-Binding Proteins, Flow Cytometry, Fungal Proteins, Gene Deletion, Gene Silencing, Green Fluorescent Proteins, Histones, Hydroxyurea, Luminescent Proteins, Molecular Chaperones, Mutation, Phenotype, Plasmids, Protein Binding, RNA, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Time Factors, Transcription, Genetic, Two-Hybrid System Techniques
Genetics
Date: Jun. 01, 2001
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