Nucleolin is involved in interferon regulatory factor-2-dependent transcriptional activation.

We have previously shown that interferon regulatory factor-2 (IRF-2) is acetylated in a cell growth-dependent manner, which enables it to contribute to the transcription of cell growth-regulated promoters. To clarify the function of acetylation of IRF-2, we investigated the proteins that associate with acetylated IRF-2. In 293T cells, the transfection ...
of p300/CBP-associated factor (PCAF) enhanced the acetylation of IRF-2. In cells transfected with both IRF-2 and PCAF, IRF-2 associated with endogenous nucleolin, while in contrast, minimal association was observed when IRF-2 was transfected with a PCAF histone acetyl transferase (HAT) deletion mutant. In a pull-down experiment using stable transfectants, acetylation-defective mutant IRF-2 (IRF-2K75R) recruited nucleolin to a much lesser extent than wild-type IRF-2, suggesting that nucleolin preferentially associates with acetylated IRF-2. Nucleolin in the presence of PCAF enhanced IRF-2-dependent H4 promoter activity in NIH3T3 cells. Nucleolin knock-down using siRNA reduced the IRF-2/PCAF-mediated promoter activity. Chromatin immunoprecipitation analysis indicated that PCAF transfection increased nucleolin binding to IRF-2 bound to the H4 promoter. We conclude that nucleolin is recruited to acetylated IRF-2, thereby contributing to gene regulation crucial for the control of cell growth.
Mesh Terms:
3T3 Cells, Acetylation, Amino Acid Substitution, Animals, Cell Line, Gene Deletion, Histone Acetyltransferases, Humans, Interferon Regulatory Factor-2, Mice, Mutation, Phosphoproteins, Promoter Regions, Genetic, RNA-Binding Proteins, Recombinant Proteins, Transcriptional Activation, Transfection, p300-CBP Transcription Factors
Oncogene
Date: Aug. 24, 2006
Download Curated Data For This Publication
170854
Switch View:
  • Interactions 3