E2A proteins enhance the histone acetyltransferase activity of the transcriptional co-activators CBP and p300.
The E2A gene encodes the E-protein transcription factors E12 and E47 that play critical roles in B-lymphopoiesis. A somatic chromosomal translocation detectable in 5% of cases of acute lymphoblastic leukemia (ALL) involves E2A and results in expression of the oncogenic transcription factor E2A-PBX1. CREB binding protein (CBP) and its close ... paralog p300 are transcriptional co-activators with intrinsic histone acetyltransferase (HAT) activity. We and others have shown that direct binding of an N-terminal transcriptional activation domain present in E12/E47 and E2A-PBX1 to the KIX domain of CBP/p300 contributes to E2A protein function. In the current work we show for the first time that the catalytic HAT activity of CBP/p300 is increased in the presence of residues 1-483 of E2A (i.e., the portion present in E2A-PBX1). The addition of purified, recombinant E2A protein to in vitro assays results in a two-fold augmentation of CBP/p300 HAT activity, whereas in vivo assays show a ten-fold augmentation of HAT-dependent transcriptional induction and a five-fold augmentation of acetylation of reporter plasmid-associated histone by CBP in response to co-transfected E2A. Our results indicate that the HAT-enhancing effect is independent of the well-documented E2A-CBP interaction involving the KIX domain and suggest a role for direct, perhaps low affinity binding of E2A to a portion of CBP that includes the HAT domain and flanking elements. Our findings add to a growing body of literature indicating that interactions between CBP/p300 and transcription factors can function in a specific manner to modulate HAT catalytic activity.
Mesh Terms:
Acetylation, Amino Acid Sequence, Basic Helix-Loop-Helix Transcription Factors, Binding Sites, CREB-Binding Protein, HEK293 Cells, Histone Acetyltransferases, Homeodomain Proteins, Humans, Oncogene Proteins, Fusion, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Transcriptional Activation, p300-CBP Transcription Factors
Acetylation, Amino Acid Sequence, Basic Helix-Loop-Helix Transcription Factors, Binding Sites, CREB-Binding Protein, HEK293 Cells, Histone Acetyltransferases, Homeodomain Proteins, Humans, Oncogene Proteins, Fusion, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Transcriptional Activation, p300-CBP Transcription Factors
Biochim. Biophys. Acta
Date: May. 01, 2012
PubMed ID: 22387215
View in: Pubmed Google Scholar
Download Curated Data For This Publication
170866
Switch View:
- Interactions 1