Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration.
Polarized cell migration is essential for normal organism development and is also a critical component of cancer cell invasion and disease progression. Directional cell motility requires the coordination of dynamic cell-extracellular matrix interactions as well as repositioning of the Golgi apparatus, both of which can be controlled by the microtubule ... (MT) cytoskeleton. In this paper, we have identified a new and conserved role for the focal adhesion scaffold protein paxillin in regulating the posttranslational modification of the MT cytoskeleton through an inhibitory interaction with the α-tubulin deacetylase HDAC6. We also determined that through HDAC6-dependent regulation of the MT cytoskeleton, paxillin regulates both Golgi organelle integrity and polarized cell invasion and migration in both three-dimensional and two-dimensional matrix microenvironments. Importantly, these data reveal a fundamental role for paxillin in coordinating MT acetylation-dependent cell polarization and migration in both normal and transformed cells.
Mesh Terms:
Acetylation, Animals, Cell Line, Tumor, Cell Movement, Cell Polarity, Extracellular Matrix, Golgi Apparatus, Histone Deacetylases, Humans, Mice, Microtubules, NIH 3T3 Cells, Paxillin, Protein Processing, Post-Translational
Acetylation, Animals, Cell Line, Tumor, Cell Movement, Cell Polarity, Extracellular Matrix, Golgi Apparatus, Histone Deacetylases, Humans, Mice, Microtubules, NIH 3T3 Cells, Paxillin, Protein Processing, Post-Translational
J. Cell Biol.
Date: Aug. 04, 2014
PubMed ID: 25070956
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