A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae.

Department of Chemistry, Auburn University, AL 36849.
A simple method is described for the direct isolation of zymosterol (5 alpha-cholesta-8,24-dien-3 beta-ol) of high purity from a sterol mutant of Saccharomyces cerevisiae. This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component.
Mesh Terms:
Cholesterol, Saccharomyces cerevisiae, Sterols
J. Steroid Biochem. Mol. Biol. Dec. 01, 1992; 43(7);741-3 [PUBMED:1472465]
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