Functional interaction between Ssu72 and the Rpb2 subunit of RNA polymerase II in Saccharomyces cerevisiae.
SSU72 is an essential gene encoding a phylogenetically conserved protein of unknown function that interacts with the general transcription factor TFIIB. A recessive ssu72-1 allele was identified as a synthetic enhancer of a TFIIB (sua7-1) defect, resulting in a heat-sensitive (Ts(-)) phenotype and a dramatic downstream shift in transcription start ... site selection. Here we describe a new allele, ssu72-2, that confers a Ts(-) phenotype in a SUA7 wild-type background. In an effort to further define Ssu72, we isolated suppressors of the ssu72-2 mutation. One suppressor is allelic to RPB2, the gene encoding the second-largest subunit of RNA polymerase II (RNAP II). Sequence analysis of the rpb2-100 suppressor defined a cysteine replacement of the phylogenetically invariant arginine residue at position 512 (R512C), located within homology block D of Rpb2. The ssu72-2 and rpb2-100 mutations adversely affected noninduced gene expression, with no apparent effects on activated transcription in vivo. Although isolated as a suppressor of the ssu72-2 Ts(-) defect, rpb2-100 enhanced the transcriptional defects associated with ssu72-2. The Ssu72 protein interacts directly with purified RNAP II in a coimmunoprecipitation assay, suggesting that the genetic interactions between ssu72-2 and rpb2-100 are a consequence of physical interactions. These results define Ssu72 as a highly conserved factor that physically and functionally interacts with the RNAP II core machinery during transcription initiation.
Mesh Terms:
Amino Acid Sequence, Carrier Proteins, Conserved Sequence, Fungal Proteins, Molecular Sequence Data, Mutation, Phosphoprotein Phosphatases, Phylogeny, Protein Subunits, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic, Transcription Factor TFIIB, Transcription Factors, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
Amino Acid Sequence, Carrier Proteins, Conserved Sequence, Fungal Proteins, Molecular Sequence Data, Mutation, Phosphoprotein Phosphatases, Phylogeny, Protein Subunits, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic, Transcription Factor TFIIB, Transcription Factors, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors
Mol. Cell. Biol.
Date: Nov. 01, 2000
PubMed ID: 11046131
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