Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae.

A tof1 mutant was recovered in a screen aimed at identifying genes involved specifically in the S phase branch of the MEC1-dependent DNA damage response pathway. The screen was based on the observation that mutants missing this branch are particularly dependent on the cell cycle-wide branch and, therefore, on RAD9, ...
for surviving DNA damage. tof1 and rad9 conferred synergistic sensitivity to MMS, UV, and HU, and the double mutant was incapable of slowing S phase in response to MMS, inducing RNR3 transcription in response to UV, and phosphorylating Rad53p in response to HU. TOF1's contribution to DNA damage response appeared to be restricted to S phase, since TOF1 did not contribute to UV-induced transcription during G1 or to the cdc13-1-induced block to anaphase in G2/M. I suggest a model in which Tof1p functions to link Mec1p with Rad53p.
Mesh Terms:
Cell Cycle Proteins, Cell Division, Cyclin B, DNA Damage, DNA-Binding Proteins, Dose-Response Relationship, Drug, Dose-Response Relationship, Radiation, Flow Cytometry, Fungal Proteins, Genotype, Hydroxyurea, Intracellular Signaling Peptides and Proteins, Methyl Methanesulfonate, Models, Genetic, Mutagens, Mutation, Protein Kinases, Protein-Serine-Threonine Kinases, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors, Transcription, Genetic, Ultraviolet Rays
Genetics
Date: Feb. 01, 2001
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