Naf1 p is a box H/ACA snoRNP assembly factor.

Box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) contain four essential proteins, Cbf5p, Gar1p, Nhp2p, and Nop10p, each of which, with the exception of Gar1p, is required for box H/ACA snoRNA accumulation. Database searches identified a novel essential protein, which we termed Naf1p, with a region of homology to the RNA-binding ...
domain of Gar1p and other features in common with hnRNP-like proteins. Naf1p is localized to the nucleus and is not a stable component of the H/ACA snoRNPs, but it is required for the accumulation of all box H/ACA snoRNAs. This requirement is not at the level of snoRNA transcription initiation or termination. Naf1 p shows in vitro RNA-binding activity and also binds directly to Cbf5p and Nhp2p. Naf1p was shown to bind to the CTD in vivo in a two-hybrid assay, and the phosphorylated CTD, but not the nonphosphorylated CTD, was shown to precipitate tagged Naf1p from a cell lysate. We propose that Naf1 p is recruited to the CTD of RNA polymerase II and binds to nascent box H/ACA snoRNAs promoting snoRNP assembly.
Mesh Terms:
Amino Acid Motifs, Amino Acid Sequence, Binding Sites, Cell Nucleus, Fungal Proteins, Hydro-Lyases, Microtubule-Associated Proteins, Molecular Sequence Data, Nuclear Proteins, RNA Polymerase II, RNA Processing, Post-Transcriptional, RNA, Ribosomal, RNA, Small Nucleolar, RNA-Binding Proteins, Recombinant Proteins, Ribonucleoproteins, Small Nuclear, Ribonucleoproteins, Small Nucleolar, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Transcription, Genetic, Yeasts
RNA
Date: Dec. 01, 2002
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