Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.

Mex67p and Mtr2p constitute an essential mRNA export complex that interacts with poly(A)+ RNA and nuclear pore proteins. We have identified Yra1p, an intranuclear protein with in vitro RNA-RNA annealing activity, which directly binds to Mex67p. The complex between Yra1p and Mex67p was reconstituted in vitro and shown by UV-crosslinking ...
to bind directly to RNA. Mutants of YRA1 are impaired in nuclear poly(A)+ RNA export at restrictive growth conditions. ALY, the mouse homologue of Yra1p and a transcriptional coactivator, can bind in vitro to yeast and human Mex67p and partly complements the otherwise non-viable yra1 null mutant. Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes.
Mesh Terms:
Amino Acid Sequence, Animals, Biological Transport, Fungal Proteins, Humans, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Poly A, Protein Binding, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Transcription Factors, Yeasts
EMBO J.
Date: Feb. 01, 2000
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