Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.
Mex67p and Mtr2p constitute an essential mRNA export complex that interacts with poly(A)+ RNA and nuclear pore proteins. We have identified Yra1p, an intranuclear protein with in vitro RNA-RNA annealing activity, which directly binds to Mex67p. The complex between Yra1p and Mex67p was reconstituted in vitro and shown by UV-crosslinking ... to bind directly to RNA. Mutants of YRA1 are impaired in nuclear poly(A)+ RNA export at restrictive growth conditions. ALY, the mouse homologue of Yra1p and a transcriptional coactivator, can bind in vitro to yeast and human Mex67p and partly complements the otherwise non-viable yra1 null mutant. Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes.
Mesh Terms:
Amino Acid Sequence, Animals, Biological Transport, Fungal Proteins, Humans, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Poly A, Protein Binding, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Transcription Factors, Yeasts
Amino Acid Sequence, Animals, Biological Transport, Fungal Proteins, Humans, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Poly A, Protein Binding, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Transcription Factors, Yeasts
EMBO J.
Date: Feb. 01, 2000
PubMed ID: 10722314
View in: Pubmed Google Scholar
Download Curated Data For This Publication
17626
Switch View:
- Interactions 1