Assessment of benzimidazole binding to individual recombinant tubulin isotypes from Haemonchus contortus.

Molecular Immunology Laboratory, School of Biomedical Sciences, Curtin University, Perth, Western Australia. moxberry@yahoo.com
One a- and 2 beta-tubulin isotypes (isotypes 1 and 2) from the parasitic nematode Haemonchus contortus were artificially expressed in E. coli and purified to obtain tubulin that was capable of polymerizing into microtubules. Binding of [14C] mebendazole (MBZ), a benzimidazole compound, to each individual unpolymerized isotype and to microtubules polymerized from recombinant alpha- and beta-tubulin was assessed and Kd and Bmax values determined. Mebendazole bound to the individual tubulin isotypes with a stoichiometry of 1:1. Binding occurred with highest affinity to alpha-tubulin followed by beta-tubulin isotype 2 and beta-tubulin isotype 1 indicating that alpha-tubulin may play a role in benzimidazole binding to microtubules. Upon polymerization of alpha- and beta-tubulin isotype 2 into microtubules the stoichiometry of binding increased to 2:1 (mebendazole : tubulin) while binding affinity remained the same. Mebendazole binding to alpha/beta-isotype 1 microtubules remained unchanged following polymerization. The increase in the number of benzimidazole receptors on alpha/beta-isotype 2 microtubules suggests the formation of a new benzimidazole receptor upon polymerization.
Mesh Terms:
Animals, Anthelmintics, Binding Sites, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Haemonchiasis, Haemonchus, Helminth Proteins, Kinetics, Mebendazole, Microtubules, Protein Isoforms, Recombinant Proteins, Tubulin
Parasitology Jun. 01, 2001; 122(0);683-7 [PUBMED:11444621]
177536
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