Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.
Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from ... the cell cycle after differentiation or serum withdrawal. The major cyclin E-associated kinase in human cells was Cdk2 (cyclin-dependent kinase 2). The abundance of the cyclin E protein and the cyclin E-Cdk2 complex was maximal in G1 cells. These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1.
Mesh Terms:
Animals, B-Lymphocytes, CDC2-CDC28 Kinases, Cell Line, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases, Cyclins, Flow Cytometry, G1 Phase, Humans, Immunoblotting, Immunosorbent Techniques, Protein Kinases, Protein-Serine-Threonine Kinases, Rats
Animals, B-Lymphocytes, CDC2-CDC28 Kinases, Cell Line, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases, Cyclins, Flow Cytometry, G1 Phase, Humans, Immunoblotting, Immunosorbent Techniques, Protein Kinases, Protein-Serine-Threonine Kinases, Rats
Science
Date: Sep. 18, 1992
PubMed ID: 1388288
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