14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae.
Deletion of the clathrin heavy-chain gene, CHC1, in the budding yeast Saccharomyces cerevisiae results in growth, morphological, and membrane trafficking defects, and in some strains chc1-delta is lethal. A previous study identified five genes which, in multicopy, rescue inviable strains of Chc- yeast. Now we report that one of the ... suppressor loci, BMH2/SCD3, encodes a protein of the 14-3-3 family. The 14-3-3 proteins are abundant acidic proteins of approximately 30 kDa with numerous isoforms and a diverse array of reported functions. The Bmh2 protein is > 70% identical to the mammalian epsilon-isoform and > 90% identical to a previously reported yeast 14-3-3 protein encoded by BMH1. Single deletions of BMH1 or BMH2 have no discernable phenotypes, but deletion of both BMH1 and BMH2 is lethal. High-copy BMH1 also rescues inviable strains of Chc- yeast, although not as well as BMH2. In addition, the slow growth of viable strains of Chc- yeast is further impaired when combined with single bmh mutations, often resulting in lethality. Overexpression of BMH genes also partially suppresses the temperature sensitivity of the cdc25-1 mutant, and high-copy TPK1, encoding a cAMP-dependent protein kinase, restores Bmh- yeast to viability. High-copy TPK1 did not rescue Chc- yeast. These genetic interactions suggest that budding-yeast 14-3-3 proteins are multifunctional and may play a role in both vesicular transport and Ras signaling pathways.
Mesh Terms:
14-3-3 Proteins, Amino Acid Sequence, Biological Transport, Clathrin, Fungal Proteins, Gene Dosage, Gene Expression Regulation, Fungal, Intracellular Membranes, Molecular Sequence Data, Mutagenesis, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Signal Transduction, Suppression, Genetic, Tyrosine 3-Monooxygenase, Ultraviolet Rays, ras Proteins
14-3-3 Proteins, Amino Acid Sequence, Biological Transport, Clathrin, Fungal Proteins, Gene Dosage, Gene Expression Regulation, Fungal, Intracellular Membranes, Molecular Sequence Data, Mutagenesis, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Signal Transduction, Suppression, Genetic, Tyrosine 3-Monooxygenase, Ultraviolet Rays, ras Proteins
Proc. Natl. Acad. Sci. U.S.A.
Date: Dec. 05, 1995
PubMed ID: 8524799
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