14-3-3 Proteins are essential for regulation of RTG3-dependent transcription in Saccharomyces cerevisiae.
14-3-3 proteins comprise a family of highly conserved proteins that bind more than 60 different, mostly phosphorylated, proteins. The yeast Saccharomyces cerevisiae has two genes, BMH1 and BMH2, encoding 14-3-3 proteins. Disruption of both genes together is lethal. In this study we constructed a mutant with a single, temperature-sensitive bmh ... allele. Recessive mutations in SIN4 and RTG3 can suppress the temperature-sensitive phenotype of this mutant. These genes encode a global transcriptional regulator and a basic helix-loop-helix transcription factor, respectively. The yeast 14-3-3 proteins were shown to bind to the Rtg3 protein. Overexpression of RTG3 is lethal even in wild-type cells. These genetic and biochemical data are consistent with a model in which the 14-3-3 proteins are required to keep the Rtg3 protein in an inactive state, which is (one of) the essential function(s) of the 14-3-3 proteins.
Mesh Terms:
14-3-3 Proteins, Antifungal Agents, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, DNA-Binding Proteins, Enzyme-Linked Immunosorbent Assay, Fungal Proteins, Gene Expression Regulation, Fungal, Mutagenesis, Polymerase Chain Reaction, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sirolimus, Transcription Factors, Tyrosine 3-Monooxygenase
14-3-3 Proteins, Antifungal Agents, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, DNA-Binding Proteins, Enzyme-Linked Immunosorbent Assay, Fungal Proteins, Gene Expression Regulation, Fungal, Mutagenesis, Polymerase Chain Reaction, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sirolimus, Transcription Factors, Tyrosine 3-Monooxygenase
Yeast
Date: Dec. 01, 2001
PubMed ID: 11748725
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