Specialization and targeting of B-type cyclins.
The B-type cyclins of S. cerevisiae are diversified with respect to time of expression during the cell cycle as well as biological function. We replaced the early-expressed CLB5 coding sequence with the late-expressed CLB2 coding sequence, at the CLB5 locus. CLB5::CLB2 exhibited almost no rescue of clb5-specific replication defects, although ... it could rescue clb1 clb2 lethality, and in synchronized cells Clb2p-associated kinase activity from CLB5::CLB2 rose early in the cell cycle, similar to that of Clb5p. Mutagenesis of a potential substrate-targeting domain of CLB5 reduced biological activity without reducing Clb5p-associated kinase activity. Thus, Clb5p may have targeting domains required for CLB5-specific biological activity.
Mesh Terms:
Cell Cycle, Cyclin B, DNA Replication, Flow Cytometry, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Lethal, Mutagenesis, Promoter Regions, Genetic, Protein Kinases, Saccharomyces cerevisiae
Cell Cycle, Cyclin B, DNA Replication, Flow Cytometry, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Lethal, Mutagenesis, Promoter Regions, Genetic, Protein Kinases, Saccharomyces cerevisiae
Mol. Cell
Date: Jul. 01, 1999
PubMed ID: 10445023
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