Hyper-resistance to nitrogen mustard in Saccharomyces cerevisiae is caused by defective choline transport.
The recessive hnm1 mutant allele is responsible for hyper-resistance to nitrogen mustard in Saccharomyces cerevisiae. Transformation with a single-copy HNM1 wild-type allele of such hyper-resistant mutants will restore wild-type sensitivity to nitrogen mustard. By contrast the presence of multi-copy vectors containing HNM1, in either a hyper-resistant hnm1 mutant or an ... HNM1 wild-type, will lead to a novel, mustard-sensitive phenotype unrelated to defects in DNA repair genes. Gene disruption of HNM1 revealed that this gene is non-essential for cells prototrophic for choline (CHO1) but lethal for cells with a cho1 genotype. Sensitivity to nitrogen mustard of wild-type HNM1, but not of hnm1 mutants, depends on the choline content of the growth medium, with cells grown in choline-free medium exhibiting the highest sensitivity. Sequencing of a 300 bp DNA fragment of HNM1 revealed the identity of this gene with the CTR locus, which is responsible for choline transport in Saccharomyces cerevisiae.
Mesh Terms:
Alleles, Biological Transport, Choline, Cloning, Molecular, DNA Damage, Drug Resistance, Microbial, Genes, Fungal, Mechlorethamine, Phenotype, Saccharomyces cerevisiae
Alleles, Biological Transport, Choline, Cloning, Molecular, DNA Damage, Drug Resistance, Microbial, Genes, Fungal, Mechlorethamine, Phenotype, Saccharomyces cerevisiae
Curr. Genet.
Date: Jun. 01, 1991
PubMed ID: 1878995
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