Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae.
The spindle checkpoint arrests cells in mitosis in response to defects in the assembly of the mitotic spindle or errors in chromosome alignment. We determined which spindle defects the checkpoint can detect by examining the interaction of mutations that compromise the checkpoint (mad1, mad2, and mad3) with those that damage ... various structural components of the spindle. Defects in microtubule polymerization, spindle pole body duplication, microtubule motors, and kinetochore components all activate the MAD-dependent checkpoint. In contrast, the cell cycle arrest caused by mutations that induce DNA damage (cdc13), inactivate the cyclin proteolysis machinery (cdc16 and cdc23), or arrest cells in anaphase (cdc15) is independent of the spindle checkpoint.
Mesh Terms:
Cell Division, Cyclins, DNA-Binding Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, cdc, Hydrolysis, Kinetochores, Microtubules, Mitotic Spindle Apparatus, Molecular Motor Proteins, Mutation, Repressor Proteins, Saccharomyces cerevisiae, Tubulin
Cell Division, Cyclins, DNA-Binding Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, cdc, Hydrolysis, Kinetochores, Microtubules, Mitotic Spindle Apparatus, Molecular Motor Proteins, Mutation, Repressor Proteins, Saccharomyces cerevisiae, Tubulin
Genetics
Date: Jun. 01, 1999
PubMed ID: 10353895
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