Role of type I myosins in receptor-mediated endocytosis in yeast.
Type I myosins are thought to drive actin-dependent membrane motility, but the direct demonstration in vivo of their involvement in specific cellular processes has been difficult. Deletion of the genes MYO3 and MYO5, which encode the yeast type I myosins, almost abolished growth. A double-deleted mutant complemented with a MYO5 ... temperature-sensitive allele (myo5-1) showed a strong defect in the internalization step of receptor-mediated endocytosis, whereas the secretory pathway remained apparently unaffected. Thus, myosin I activity is required for a budding event in endocytosis but not for several other aspects of membrane traffic.
Mesh Terms:
Alleles, Endocytosis, Gene Deletion, Molecular Sequence Data, Mutation, Myosins, Peptides, Receptors, Mating Factor, Receptors, Peptide, Saccharomyces cerevisiae, Temperature, Transcription Factors
Alleles, Endocytosis, Gene Deletion, Molecular Sequence Data, Mutation, Myosins, Peptides, Receptors, Mating Factor, Receptors, Peptide, Saccharomyces cerevisiae, Temperature, Transcription Factors
Science
Date: Apr. 26, 1996
PubMed ID: 8614799
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