Two mechanisms of butenafine action in Candida albicans.
The mechanism of action of a new benzylamine antimycotic, butenafine hydrochloride, was studied in Candida albicans by using the thiocarbamate antimycotic tolnaftate as a reference drug. Butenafine completely inhibited the growth of a test strain of C. albicans at 25 micrograms/ml and was cidal at 50 micrograms/ml. Tolnaftate did not ... show any growth-inhibitory activity up to 100 micrograms/ml. Both butenafine and tolnaftate inhibited squalene epoxidation in C. albicans, with 50% inhibitory concentrations being 0.57 and 0.17 microgram/ml, respectively. Butenafine, but not tolnaftate, induced the release of appreciable amounts of Pi from C. albicans cells at 12.5 micrograms/ml. This effect of butenafine was augmented when the cells were pretreated with tolnaftate. The results suggest that the direct membrane-damaging effect of butenafine may play a major role in its anticandidal activity and that the drug-induced alteration in the cellular sterol composition renders the cell membrane more susceptible to the membrane-damaging effect of this drug.
Mesh Terms:
Acetates, Antifungal Agents, Benzylamines, Candida albicans, Chromatography, Gas, Chromatography, Thin Layer, Lipid Metabolism, Lipids, Microbial Sensitivity Tests, Naphthalenes, Tolnaftate
Acetates, Antifungal Agents, Benzylamines, Candida albicans, Chromatography, Gas, Chromatography, Thin Layer, Lipid Metabolism, Lipids, Microbial Sensitivity Tests, Naphthalenes, Tolnaftate
Antimicrob. Agents Chemother.
Date: Apr. 01, 1993
PubMed ID: 8494375
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