Mutations in the gene encoding the 34 kDa subunit of yeast replication protein A cause defective S phase progression.

The in vivo function of the 34 kDa subunit of yeast replication protein A (RPA), encoded by the RFA2 gene, has been studied by analyzing the effect of Rpa34 depletion and by producing and characterizing rfa2 temperature-sensitive mutants. We show that unbalanced stoichiometry of the RPA subunits does not affect ...
cell growth and cell cycle progression until the level of Rpa34 becomes rate-limiting, at which point cells arrest with a late S/G2 DNA content. Rpa34 is involved in DNA replication in vivo, since rfa2 ts mutants are defective in S phase progression and ARS plasmid stability, and rfa2 pol1 double mutants are non-viable. Moreover, when shifted to the restrictive temperature, about 50% of the rfa2 mutant cells rapidly die while traversing the S phase and the surviving cells arrest in late S/G2 at the RAD9 checkpoint. Finally, rfa2 mutant cells have a mutator and hyper-recombination phenotype and are more sensitive to hydroxyurea and methyl-methane-sulfonate than wild-type cells.
Mesh Terms:
Bacterial Proteins, Base Sequence, Cell Cycle, Cell Cycle Proteins, Cell Death, Cell Division, DNA Polymerase II, DNA Repair, DNA Replication, DNA-Binding Proteins, Fungal Proteins, G2 Phase, Glycosyltransferases, Molecular Sequence Data, Mutation, Plasmids, Replication Protein A, Repressor Proteins, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factors
J. Mol. Biol.
Date: Dec. 08, 1995
Download Curated Data For This Publication
18018
Switch View:
  • Interactions 1