Diazoxide suppresses slowly-inactivating outward and inward currents in CA1 hippocampal neurones.
Diazoxide (DZX) opens ATP-sensitive K (KATP) channels in muscle and other cells. In whole-cell voltage-clamp recordings in slices, in the presence of kynurenate and bicuculline to minimize indirect effects, DZX (0.65 mM) did not increase input conductance; but it sharply reduced persistent inward and outward currents. An inward current (peak ... near -20 mV) was especially clear in the presence of K channel blockers; was fully evident in Ca-channel blocking medium; but was abolished by tetrodotoxin. The main direct effects of DZX on these neurones are thus mediated not by activation of KATP channels, but rather by modulation of voltage-dependent channels, including a TTX-sensitive persistent NA current and possibly a Ca current.
Mesh Terms:
Animals, Calcium, Calcium Channel Blockers, Diazoxide, Evoked Potentials, Hippocampus, In Vitro Techniques, Ion Channel Gating, Neurons, Potassium Channels, Rats, Rats, Sprague-Dawley, Sodium Channels, Tetrodotoxin
Animals, Calcium, Calcium Channel Blockers, Diazoxide, Evoked Potentials, Hippocampus, In Vitro Techniques, Ion Channel Gating, Neurons, Potassium Channels, Rats, Rats, Sprague-Dawley, Sodium Channels, Tetrodotoxin
Neuroreport
Date: Dec. 13, 1993
PubMed ID: 8298083
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