Requirement of yeast SGS1 and SRS2 genes for replication and transcription.
The SGS1 gene of the yeast Saccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion of SGS1 and SRS2 is lethal in yeast. Here, using a ... conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase I transcription are drastically inhibited in the srs2Delta sgs1-ts strain at the restrictive temperature. Thus, SGS1 and SRS2 function in DNA replication and RNA polymerase I transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.
Mesh Terms:
Bloom Syndrome, Codon, DNA Helicases, DNA Replication, DNA, Fungal, Fungal Proteins, Gene Deletion, Genes, Fungal, Humans, Mutation, RNA Polymerase I, RNA Polymerase II, RNA Polymerase III, RNA, Fungal, RNA, Messenger, RNA, Ribosomal, RNA, Transfer, Amino Acid-Specific, RecQ Helicases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription, Genetic, Werner Syndrome
Bloom Syndrome, Codon, DNA Helicases, DNA Replication, DNA, Fungal, Fungal Proteins, Gene Deletion, Genes, Fungal, Humans, Mutation, RNA Polymerase I, RNA Polymerase II, RNA Polymerase III, RNA, Fungal, RNA, Messenger, RNA, Ribosomal, RNA, Transfer, Amino Acid-Specific, RecQ Helicases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription, Genetic, Werner Syndrome
Science
Date: Dec. 17, 1999
PubMed ID: 10600744
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