The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5.

Snf/Swi, a nucleosome remodeling complex, is important for overcoming nucleosome-mediated repression of transcription in Saccharomyces cerevisiae. We have addressed the mechanism by which Snf/Swi controls transcription in vivo of an Snf/Swi-dependent promoter, that of the SUC2 gene. By single-cell analysis, our results show that Snf/Swi is required for activated levels ...
of SUC2 expression in every cell of a population. In addition, Snf/Swi is required for maintenance of SUC2 transcription, suggesting that continuous chromatin remodeling is necessary to maintain an active transcriptional state. Finally, Snf/Swi and Gcn5, a histone acetyltransferase, have partially redundant roles in the control of SUC2 transcription, suggesting a functional overlap between two different mechanisms believed to overcome repression by nucleosomes, nucleosome remodeling and histone acetylation.
Mesh Terms:
Acetyltransferases, Carrier Proteins, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Epistasis, Genetic, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Reporter, Green Fluorescent Proteins, Histone Acetyltransferases, Luminescent Proteins, Membrane Transport Proteins, Models, Genetic, Molecular Conformation, Mutation, Nucleosomes, Plant Proteins, Protein Kinases, Protein-Serine-Threonine Kinases, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Templates, Genetic, Transcription Factors, Transcription, Genetic
EMBO J.
Date: Jun. 01, 1999
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