HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae.

To investigate cell cycle regulation at the S or G2 phase in Saccharomyces cerevisiae, we have isolated mutants displaying supersensitivity to hydroxyurea (HU), a chemical that inhibits DNA replication. Such mutants, which we have named hydroxyurea sensitive (hys), defined four linkage groups and we characterized the hys2 mutation in this ...
study. The hys2-1 mutant displays temperature sensitive growth and a constellation of phenotypes indicating defective DNA metabolism. At the restrictive temperature, hys2-1 cells arrest as large budded cells with a single nucleus at the neck of the bud and a short spindle. The hys2-1 mutant exhibits increased rates of chromosome loss and recombination. Additionally, hys2-1 appears to accumulate incompletely replicated DNA that can be detected by a pulse field electrophoresis assay. Finally, deletion of RAD9 in a hys2-1 strain decreases the percentage of arrested cells, suggesting that an intact RAD9-checkpoint is required for the cell cycle arrest in hys2-1 cells. HYS2 encodes a 55 kDa protein that is essential for viability at all temperatures. Taken together, these data suggest that Hys2 plays a role in DNA replication.
Mesh Terms:
Base Sequence, Cell Cycle, Cell Cycle Proteins, Cloning, Molecular, DNA Polymerase III, DNA Primers, DNA Replication, DNA, Fungal, DNA-Directed DNA Polymerase, Enzyme Inhibitors, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Hydroxyurea, Molecular Sequence Data, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Nucleic Acids Res.
Date: Sep. 11, 1995
Download Curated Data For This Publication
Switch View:
  • Interactions 1