HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae.
To investigate cell cycle regulation at the S or G2 phase in Saccharomyces cerevisiae, we have isolated mutants displaying supersensitivity to hydroxyurea (HU), a chemical that inhibits DNA replication. Such mutants, which we have named hydroxyurea sensitive (hys), defined four linkage groups and we characterized the hys2 mutation in this ... study. The hys2-1 mutant displays temperature sensitive growth and a constellation of phenotypes indicating defective DNA metabolism. At the restrictive temperature, hys2-1 cells arrest as large budded cells with a single nucleus at the neck of the bud and a short spindle. The hys2-1 mutant exhibits increased rates of chromosome loss and recombination. Additionally, hys2-1 appears to accumulate incompletely replicated DNA that can be detected by a pulse field electrophoresis assay. Finally, deletion of RAD9 in a hys2-1 strain decreases the percentage of arrested cells, suggesting that an intact RAD9-checkpoint is required for the cell cycle arrest in hys2-1 cells. HYS2 encodes a 55 kDa protein that is essential for viability at all temperatures. Taken together, these data suggest that Hys2 plays a role in DNA replication.
Mesh Terms:
Base Sequence, Cell Cycle, Cell Cycle Proteins, Cloning, Molecular, DNA Polymerase III, DNA Primers, DNA Replication, DNA, Fungal, DNA-Directed DNA Polymerase, Enzyme Inhibitors, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Hydroxyurea, Molecular Sequence Data, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Base Sequence, Cell Cycle, Cell Cycle Proteins, Cloning, Molecular, DNA Polymerase III, DNA Primers, DNA Replication, DNA, Fungal, DNA-Directed DNA Polymerase, Enzyme Inhibitors, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Hydroxyurea, Molecular Sequence Data, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Nucleic Acids Res.
Date: Sep. 11, 1995
PubMed ID: 7567461
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