Allele-specific interactions between the yeast RFC1 and RFC5 genes suggest a basis for RFC subunit-subunit interactions.
Replication factor C (RFC) is an essential, multi-subunit ATPase that functions in DNA replication, DNA repair, and DNA metabolism-related checkpoints. In order to investigate how the individual RFC subunits contribute to these functions in vivo, we undertook a genetic analysis of RFC genes from budding yeast. We isolated and characterized ... mutations in the RFC5 gene that could suppress the cold-sensitive phenotype of rfc1-1 mutants. Analysis of the RFC5 suppressors revealed that they could not suppress the elongated telomere phenotype, the sensitivity to DNA damaging agents, or the mutator phenotype of rfc1-1 mutants. Unlike the checkpoint-defective rfc5-1 mutation, the RFC5 suppressor mutations did not interfere with the methylmethane sulfonate- or hydroxyurea-induced phosphorylation of Rad53p. The Rfc5p suppressor substitutions mapped to amino acid positions in the conserved RFC box motifs IV-VII. Comparisons of the structures of related RFC box-containing proteins suggest that these RFC motifs may function to coordinate interactions between neighboring subunits of multi-subunit ATPases.
Mesh Terms:
Alleles, Amino Acid Motifs, Amino Acid Sequence, Cell Cycle Proteins, DNA Damage, DNA Replication, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Homeodomain Proteins, Hydroxyurea, Methyl Methanesulfonate, Models, Molecular, Molecular Sequence Data, Mutation, Phenotype, Protein Structure, Quaternary, Protein Subunits, Proto-Oncogene Proteins c-bcl-2, Replication Protein C, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Suppression, Genetic, Telomere
Alleles, Amino Acid Motifs, Amino Acid Sequence, Cell Cycle Proteins, DNA Damage, DNA Replication, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Homeodomain Proteins, Hydroxyurea, Methyl Methanesulfonate, Models, Molecular, Molecular Sequence Data, Mutation, Phenotype, Protein Structure, Quaternary, Protein Subunits, Proto-Oncogene Proteins c-bcl-2, Replication Protein C, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Suppression, Genetic, Telomere
Mol. Gen. Genet.
Date: Nov. 01, 2000
PubMed ID: 11129041
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