Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae.
A temperature-sensitive mutation (act1-1) in the essential actin gene of Saccharomyces cerevisiae can be suppressed by mutations in the SAC2 gene. A cloned genomic DNA fragment that complements the cold-sensitive growth phenotype associated with such a suppressor mutation (sac2-1) was sequenced. The fragment contained an open reading frame that encodes ... a 641 amino acid predicted hydrophilic protein with a molecular weight of 74,445. No sequences with significant similarity to SAC2 were found in the GenBank and EMBL databases. A SAC2 disruption mutation was constructed which had phenotypes similar to the sac2-1 point mutation. A haploid SAC2 disruption strain failed to grow at low temperature and the disruption allele suppressed the temperature-sensitive act1-1 growth defect. The suppression phenotype was dependent on the strain background.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Chromosome Mapping, Chromosomes, Fungal, Cloning, Molecular, Fungal Proteins, Gene Deletion, Genes, Fungal, Genetic Markers, Genetic Vectors, Molecular Sequence Data, Mutation, Phenotype, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Amino Acid Sequence, Base Sequence, Chromosome Mapping, Chromosomes, Fungal, Cloning, Molecular, Fungal Proteins, Gene Deletion, Genes, Fungal, Genetic Markers, Genetic Vectors, Molecular Sequence Data, Mutation, Phenotype, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Yeast
Date: Sep. 01, 1994
PubMed ID: 7754710
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