Zizimin1, a novel Cdc42 activator, reveals a new GEF domain for Rho proteins.
The Rho family GTPases Rac, Rho and Cdc42 are critical in regulating the actin-based cytoskeleton, cell migration, growth, survival and gene expression. These GTPases are activated by guanine nucleotide-exchange factors (GEFs). A biochemical search for Cdc42 activators led to the cloning of zizimin1, a new protein whose overexpression induces Cdc42 ... activation. Sequence comparison combined with mutational analysis identified a new domain, which we named CZH2, that mediates direct interaction with Cdc42. CZH2-containing proteins constitute a new superfamily that includes the so-called 'CDM' proteins that bind to and activate Rac. Together, the results suggest that CZH2 is a new GEF domain for the Rho family of proteins.
Mesh Terms:
3T3 Cells, Amino Acid Sequence, Animals, Binding Sites, Cloning, Molecular, Enzyme Activation, Guanine Nucleotide Exchange Factors, Humans, Mice, Molecular Sequence Data, Protein Structure, Tertiary, RNA, Messenger, Sequence Homology, Amino Acid, Tissue Distribution, cdc42 GTP-Binding Protein, rho GTP-Binding Proteins
3T3 Cells, Amino Acid Sequence, Animals, Binding Sites, Cloning, Molecular, Enzyme Activation, Guanine Nucleotide Exchange Factors, Humans, Mice, Molecular Sequence Data, Protein Structure, Tertiary, RNA, Messenger, Sequence Homology, Amino Acid, Tissue Distribution, cdc42 GTP-Binding Protein, rho GTP-Binding Proteins
Nat. Cell Biol.
Date: Sep. 01, 2002
PubMed ID: 12172552
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