RIP3 and AtKinesin-13A - a novel interaction linking Rho proteins of plants to microtubules.
RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We ... found that RIP3 has properties and cellular roles different from the previously described RIP family member ICR1/RIP1. We show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth.
Mesh Terms:
Arabidopsis, Arabidopsis Proteins, Calorimetry, Chromatography, Gel, Fluorescence Resonance Energy Transfer, Microtubules, Protein Binding, Two-Hybrid System Techniques
Arabidopsis, Arabidopsis Proteins, Calorimetry, Chromatography, Gel, Fluorescence Resonance Energy Transfer, Microtubules, Protein Binding, Two-Hybrid System Techniques
Eur. J. Cell Biol.
Date: Dec. 01, 2010
PubMed ID: 20832900
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