Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein.
The SSN6-TUP1 protein complex represses transcription of diversely regulated genes in the yeast Saccharomyces cerevisiae. Here we present evidence that MIG1, a zinc-finger protein in the EGR1/Zif268 family, recruits SSN6-TUP1 to glucose-repressed promoters. DNA-bound LexA-MIG1 represses transcription of a target gene in glucose-grown cells, and repression requires SSN6 and TUP1. ... We also show that MIG1 and SSN6 fusion proteins interact in the two-hybrid system. Unexpectedly, we found that LexA-MIG1 activates transcription strongly in an ssn6 mutant and weakly in a tup1 mutant. Finally, LexA-MIG1 does not repress transcription in glucose-deprived cells, and MIG1 is differentially phosphorylated in response to glucose availability. We suggest a role for phosphorylation in regulating repression.
Mesh Terms:
Bacterial Proteins, Base Sequence, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Glucose, Models, Genetic, Molecular Sequence Data, Nuclear Proteins, Phosphorylation, Protein Binding, Recombinant Fusion Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Serine Endopeptidases, Transcription, Genetic
Bacterial Proteins, Base Sequence, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Glucose, Models, Genetic, Molecular Sequence Data, Nuclear Proteins, Phosphorylation, Protein Binding, Recombinant Fusion Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Serine Endopeptidases, Transcription, Genetic
Proc. Natl. Acad. Sci. U.S.A.
Date: Apr. 11, 1995
PubMed ID: 7724528
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