Foerster resonance energy transfer demonstrates a flavonoid metabolon in living plant cells that displays competitive interactions between enzymes.

We have used Foerster resonance energy transfer detected by fluorescence lifetime imaging microscopy (FLIM-FRET) to provide the first evidence from living plants cells for the existence of a flavonoid metabolon. The distribution of flux within this system may be regulated by the direct competition of enzymes that catalyze key branch-point ...
reactions, flavonol synthase 1 and dihydroflavonol 4-reductase, for association with the entry-point enzyme, chalcone synthase. Because the flavonoid enzymes were likely recruited from pathways of primary metabolism, our findings suggest a new general working model for the regulation of dynamic pathways in their native cellular context.
Mesh Terms:
Arabidopsis, Arabidopsis Proteins, Flavonoids, Fluorescence Resonance Energy Transfer, Microscopy, Fluorescence
FEBS Lett.
Date: Jul. 21, 2011
Download Curated Data For This Publication
185165
Switch View:
  • Interactions 3