Complex formation between RAS and RAF and other protein kinases.
We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 ... and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.
Mesh Terms:
Base Sequence, Fungal Proteins, GTP-Binding Proteins, Guanosine Triphosphate, MAP Kinase Kinase 1, Mitogen-Activated Protein Kinase Kinases, Molecular Sequence Data, Protein Kinases, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, Proto-Oncogene Proteins p21(ras), Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, ras Proteins
Base Sequence, Fungal Proteins, GTP-Binding Proteins, Guanosine Triphosphate, MAP Kinase Kinase 1, Mitogen-Activated Protein Kinase Kinases, Molecular Sequence Data, Protein Kinases, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, Proto-Oncogene Proteins p21(ras), Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, ras Proteins
Proc. Natl. Acad. Sci. U.S.A.
Date: Jul. 01, 1993
PubMed ID: 8327501
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