Transcriptional activating regions target a cyclin-dependent kinase.
Several yeast activators are phosphorylated by SRB10, a cyclin-dependent kinase associated with the transcriptional machinery. Sites of phosphorylation are found outside the activating region in each case, and the modification has different physiological consequences in different cases. We show here that certain acidic transcriptional activating regions contact SRB10 as assayed ... both in vivo and in vitro. The interaction evidently positions each activator, as it activates transcription, so that it gets phosphorylated by SRB10, and thus a common mechanism targets disparate substrates to the kinase.
Mesh Terms:
Cyclin-Dependent Kinase 8, Cyclin-Dependent Kinases, DNA-Binding Proteins, Protein Biosynthesis, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Surface Plasmon Resonance, Transcription Factors, Transcription, Genetic, Transcriptional Activation
Cyclin-Dependent Kinase 8, Cyclin-Dependent Kinases, DNA-Binding Proteins, Protein Biosynthesis, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Surface Plasmon Resonance, Transcription Factors, Transcription, Genetic, Transcriptional Activation
Proc. Natl. Acad. Sci. U.S.A.
Date: Nov. 12, 2002
PubMed ID: 12417740
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