[Identification of proteins interacted with Bat3 using tandem affinity purification].

To identify the specific protein interactions involved in Bat3-mediated apoptosis.Tandem affinity purification (TAP) was utilized to investigate Bat3-protein interactions, during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions. The isolated proteins were identified with mass spectrometry.TAP studies ...
showed that Ubl4A was identified as a Bat3-binding partner. Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.TAP was successfully established and is suitable for isolating the binding partners of Bat3.
Mesh Terms:
Cell Line, Humans, Molecular Chaperones, Protein Binding, Ubiquitins
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Date: Feb. 01, 2014
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