Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.
Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the ... heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.
Mesh Terms:
Binding Sites, Cell Cycle Proteins, Green Fluorescent Proteins, HeLa Cells, Heat-Shock Response, Heterogeneous-Nuclear Ribonucleoprotein Group M, Humans, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, RNA Splice Sites, RNA-Binding Proteins, Recombinant Fusion Proteins
Binding Sites, Cell Cycle Proteins, Green Fluorescent Proteins, HeLa Cells, Heat-Shock Response, Heterogeneous-Nuclear Ribonucleoprotein Group M, Humans, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, RNA Splice Sites, RNA-Binding Proteins, Recombinant Fusion Proteins
EMBO Rep.
Date: Jun. 01, 2010
PubMed ID: 20467437
View in: Pubmed Google Scholar
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