Avoidance of ribonucleotide-induced mutations by RNase H2 and Srs2-Exo1 mechanisms.

Srs2 helicase is known to dismantle nucleofilaments of Rad51 recombinase to prevent spurious recombination events and unwind trinucleotide sequences that are prone to hairpin formation. Here we document a new, unexpected genome maintenance role of Srs2 in the suppression of mutations arising from mis-insertion of ribonucleoside monophosphates during DNA replication. ...
In cells lacking RNase H2, Srs2 unwinds DNA from the 5' side of a nick generated by DNA topoisomerase I at a ribonucleoside monophosphate residue. In addition, Srs2 interacts with and enhances the activity of the nuclease Exo1, to generate a DNA gap in preparation for repair. Srs2-Exo1 thus functions in a new pathway of nick processing-gap filling that mediates tolerance of ribonucleoside monophosphates in the genome. Our results have implications for understanding the basis of Aicardi-Goutieres syndrome, which stems from inactivation of the human RNase H2 complex.
Mesh Terms:
Animals, Cell Line, DNA Damage, DNA Helicases, Escherichia coli, Exodeoxyribonucleases, Genomic Instability, Mutation, Ribonuclease H, Ribonucleotides, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Nature
Date: Jul. 10, 2014
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