Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death.

Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. ...
This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects.
Mesh Terms:
Apoptosis, Cytosol, HeLa Cells, Humans, Oligopeptides, Paclitaxel, Protein Kinase C-delta
FEBS Lett.
Date: Apr. 24, 2012
Download Curated Data For This Publication
187104
Switch View:
  • Interactions 3