Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF).

Interleukin 17 (IL-17) promotes the expression of chemokines and cytokines via the induction of gene transcription and post-transcriptional stabilization of mRNA. We show here that IL-17 enhanced the stability of chemokine CXCL1 mRNA and other mRNAs through a pathway that involved the adaptor Act1, the adaptors TRAF2 or TRAF5 and the splicing factor SF2 (also known as alternative splicing factor (ASF)). TRAF2 and TRAF5 were necessary for IL-17 to signal the stabilization of CXCL1 mRNA. Furthermore, IL-17 promoted the formation of complexes of TRAF5-TRAF2, Act1 and SF2 (ASF). Overexpression of SF2 (ASF) shortened the half-life of CXCL1 mRNA, whereas depletion of SF2 (ASF) prolonged it. SF2 (ASF) bound chemokine mRNA in unstimulated cells, whereas the SF2 (ASF)-mRNA interaction was much lower after stimulation with IL-17. Our findings define an IL-17-induced signaling pathway that links to the stabilization of selected mRNA species through Act1, TRAF2-TRAF5 and the RNA-binding protein SF2 (ASF).
Mesh Terms:
Adaptor Proteins, Signal Transducing, Alternative Splicing, Animals, Chemokine CXCL1, Female, Half-Life, HeLa Cells, Humans, Inflammation, Interleukin-17, Mice, Mice, Knockout, Nuclear Proteins, RNA Processing, Post-Transcriptional, RNA Stability, RNA, Messenger, RNA-Binding Proteins, Signal Transduction, TNF Receptor-Associated Factor 5, Th17 Cells, Transcription, Genetic
Nat. Immunol. Sep. 01, 2011; 12(9);853-60 [PUBMED:21822258]
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