Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF).

Interleukin 17 (IL-17) promotes the expression of chemokines and cytokines via the induction of gene transcription and post-transcriptional stabilization of mRNA. We show here that IL-17 enhanced the stability of chemokine CXCL1 mRNA and other mRNAs through a pathway that involved the adaptor Act1, the adaptors TRAF2 or TRAF5 and ...
the splicing factor SF2 (also known as alternative splicing factor (ASF)). TRAF2 and TRAF5 were necessary for IL-17 to signal the stabilization of CXCL1 mRNA. Furthermore, IL-17 promoted the formation of complexes of TRAF5-TRAF2, Act1 and SF2 (ASF). Overexpression of SF2 (ASF) shortened the half-life of CXCL1 mRNA, whereas depletion of SF2 (ASF) prolonged it. SF2 (ASF) bound chemokine mRNA in unstimulated cells, whereas the SF2 (ASF)-mRNA interaction was much lower after stimulation with IL-17. Our findings define an IL-17-induced signaling pathway that links to the stabilization of selected mRNA species through Act1, TRAF2-TRAF5 and the RNA-binding protein SF2 (ASF).
Mesh Terms:
Adaptor Proteins, Signal Transducing, Alternative Splicing, Animals, Chemokine CXCL1, Female, Half-Life, HeLa Cells, Humans, Inflammation, Interleukin-17, Mice, Mice, Knockout, Nuclear Proteins, RNA Processing, Post-Transcriptional, RNA Stability, RNA, Messenger, RNA-Binding Proteins, Signal Transduction, TNF Receptor-Associated Factor 5, Th17 Cells, Transcription, Genetic
Nat. Immunol.
Date: Sep. 01, 2011
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